Scientist

Payrate: $40.00-$42.00/hr

Job Description:

We are seeking a highly skilled and motivated contract worker to join our protein engineering team. The ideal candidate will have extensive experience in cell maintenance and expansion, transfection, and protein expression in mammalian cells. This role requires proficiency in making buffers, protein purification chromatography (with a strong emphasis on affinity chromatography and AKTA experience), buffer exchange endotoxin measurement and analytics, aliquoting and inventory management.

Key Responsibilities:

• Cell Maintenance and Expansion: Perform routine maintenance and expansion of mammalian cell cultures to ensure optimal growth and viability.
• Transfection and Protein Expression: Transfect mammalian cell cultures for antibody/protein expression in both flasks & 96 well blocks. Track titers, troubleshoot.
• Buffer Preparation: Prepare various buffers required for protein purification and other laboratory processes.
• Protein Purification Chromatography: Execute protein purification protocols, with a focus on affinity chromatography using AKTA and Kingfisher systems.
• Buffer Exchange: Perform buffer exchange procedures, particularly using Big Tuna systems.
• Endotoxin Measurement and Cleanup: Measure and remove endotoxins from protein samples to ensure purity and safety.
• Aliquoting and Inventory Management: Aliquoting and Inventory Management: Accurately aliquot protein samples, manage inventory, and handle shipments to maintain an organized and efficient laboratory environment.

Qualifications:

• Bachelor's or Master’s degree in Molecular Biology, Biochemistry, or a related field.
• Proven experience in cell culture maintenance and expansion.
• Hands-on experience with transfection and protein expression in mammalian cells.
• Proficiency in making buffers and performing protein purification chromatography, especially affinity chromatography with AKTA systems.
• Experience with buffer exchange techniques; Experience with Big Tuna instrument is a plus.
• Knowledge of endotoxin measurement and cleanup procedures.
• Strong organizational skills and attention to detail for aliquoting, inventory management, eln entry, and managing shipments.
• Experience with protein analytics (capillary electrophoresis, Octet Quantification, stability studies) are a plus
• Excellent communication and teamwork skills.
• Previous experience in a contract or temporary role within a biotechnology or pharmaceutical company is preferred.

Top 3 Must Have Skill Sets:

• Mammalian Protein Expression
• Affinity Chromatography (Purification)
• Excellent Recordkeeping/ELN/Inventory skills

Pay Transparency:

The typical base pay for this role across the U.S. is: $40.00-$42.00/hr /hr /hr. Final offer amounts, within the base pay set forth above, are determined by factors including your relevant skills, education and experience and the benefits package you select. Full-time employees are eligible to select from different benefits packages. Packages may include medical, dental, and vision benefits, 10 paid days off, 401(k) plan participation, commuter benefits and life and disability insurance.

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Aditi Consulting LLC uses AI technology to engage candidates during the sourcing process. AI technology is used to gather data only and does not replace human-based decision making in employment decisions. By applying to this position, you agree to Aditi’s use of AI technology including calls from an AI Voice Recruiter.

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