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Manufacturing Operator

EnPlusOne Biosciences
Watertown, MA Full Time
POSTED ON 3/27/2025
AVAILABLE BEFORE 4/24/2025

About EnPlusOne

EnPlusOne is enabling the future of RNA. Its ezRNA™ synthesis platform is a revolutionary innovation that uses enzymes to make therapeutic RNA in an aqueous, sustainable manner. Our mission is to solve the issues in traditional chemical synthesis and large-scale manufacturing, and usher in a new wave of RNA therapeutics. We are a team of biologists, chemists, biochemists, and engineers working together to make better RNA enzymatically. For more information, visit

Role & Responsibilities

Your primary role as a Manufacturing Operator is to produce enzymes to advance our ezRNA™ synthesis platform. You will work closely with staff scientists and process engineers to produce, purify, and analyze EnPlusOne’s proprietary enzymes and meet production targets in support of key technical milestones and projects. Additional responsibilities include thorough documentation, recording observations, troubleshooting mechanical issues, proposing process improvements, and preparing quality control reports. You will also be an instrumental part in EnPlusOne’s expansion and scale up goals in 2025. Successful candidates are motivated, curious, flexible, and able to collaborate and communicate in a multidisciplinary environment.

Qualifications

  • High-school diploma or BS in related field
  • At least 3 years of experience in a manufacturing or operations role in the biotechnology or pharmaceutical industry
  • Preference for experience in cGMP operations and GDP

Desired Skills

  • Experienced in buffer preparation including weigh and dispense, liquid additions, pH adjustment, and applicable quality control tests (i.e. pH and conductivity)
  • Experience operating standard protein purification unit operations including : bioreactors, TFF / MF, chromatography, UF / DF, and nanofiltration
  • Experience with chromatography support operations including resin slurry preparation, column packing, and column qualification
  • Experience operating Cytiva AKTA Pure systems and familiarity with UNICORN software is beneficial, however, not required
  • Experience operating pumps, filtration, and performing filter integrity testing (FIT)
  • Demonstrated ability for project management, digital record keeping, and maintenance of SOPs
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