What are the responsibilities and job description for the In Vivo Scientist position at Garuda Therapeutics, Inc.?
The successful candidate will have outstanding expertise in mouse handling and mouse procedures. They will be responsible for managing mouse colonies, performing project-specific in vivo experiments, intravenous injections of hematopoietic stem cells in humanized murine models, retro-orbital and submandibular blood collection, and blood cell FACS analyses and in vivo imaging. This is a collaborative role that will be partnering with other team members to assist with project-specific- in vivo experiments as well as other needs across the process development function.
Role and Responsibilities:
- Hands-on role with a commitment to designing, conducting and analyzing in vivo studies to assess engraftment of hematopoietic stem cells.
- Execute pluripotent stem cell culture and differentiation into hematopoietic stem cells to support process development, optimization, and characterization.
- Document experimental procedures and results according to established guidelines; Perform statistical analysis of experimental data.
- Collaborate effectively within departmental teams, engage in team discussions to resolve issues, and lead projects with a fully integrated team to facilitate successful outcomes.
- Prior experience in bioreactor-based process development for iPSC-derived cell therapies is highly desirable.
Qualifications:
- Advanced degree (M.S. or Ph.D.) in molecular biology or pharmacology, with at least 2 years of industry experience is required
- Experience working with human induced pluripotent stem cells (iPSC), hematopoietic stem cells (HSC), and/or immune cell biology; including scale-up processes utilizing bioreactors such as PBS vertical wheel, Rotea counterflow centrifuge is required
- Proficiency with in-vivo techniques including animal handling, intra-bone and intravenous transplants, blood collection and tissue dissection
- Experience in standard processing techniques including flow cytometry, molecular biology assays such as CFUs, immunological assays, and aseptic cell culture technique is required
- Experience with experiment design tools such as FlowJo and practical knowledge of statistical analysis is required
- Strong organizational, analytical, problem-solving, root-cause-analysis, and trouble-shooting skills are required
