Scientist

Title: Research Scientist

Location: San Diego, CA

Duration: 6 months

Top Skills:

• Purification and characterization of bispecific antibodies.
• Experience with complex modalities.
• Automated liquid handling experience a plus.

Job Description:

• The successful candidate will have knowledge in purification and characterization of bispecific antibodies and will be familiar with state-of-the-art methods to support biotherapeutic discovery efforts.
• The candidate will have demonstrated success in purification of antibodies and complex molecules, executing challenging multi-step purification, analytical data interpretation, troubleshooting and formulation of final product.
• Experience in automation of high-throughput purification is a plus.
• The candidate will show an independent ability to design purification methods, understand biochemical concepts and interpret data to generate and improve design.

Core Job Functions:

• Primary responsibility is to perform original research to develop marketable products.
• Special emphasis will be placed on automated antibody and bispecific purification, which will involve working with GE AKTA (Pure/Avant) and Unicorn software.
• Knowledge of Protein Maker instrument a plus.
• Generate mg to gram quantities of material for in-vivo efficacy studies.
• Develop and optimize high-throughput purification capabilities using Lynx900 and KingFisher Flex System.
• Packing of chromatography columns, operating endotoxin free chromatography, including operating preparative (FPLC) and analytical (HPLC) chromatography systems.
• Analytical characterization including endotoxin measurements, protein quantitation assays, analytical HPLC chromatography (HIC, SEC), capillary electrophoresis (CE-SDS and cIEF), DLS, and mass photometry.
• Buffer preparation, molar concentration quantitation and pH adjustment.
• Storing, inventorying, and ordering department supplies.
• Participates in cross-functional teams by performing assigned experiments and offering technical advice at team meetings; shares technical expertise with other colleagues; trains or provides guidance to other personnel, if needed.

Basic Qualification:

• Bachelor's Degree 5 years of academic / industry experience.
• Master's degree 3 years of academic / industry experience.

Preferred Qualifications:

• Experience in antibody/bi-specific purification and characterization including, but not limited to SDS-PAGE, CE-SDS, CiEF, AnSEC, endotoxin measurement, A280.
• Automated liquid handling a plus.
• Sample inventory management.