Validation Lab Technician - Laboratory

Validation Lab Technician
Medical Diagnostic Laboratories (MDL) is a CLIA certified clinical laboratory with multiple state licensing, specializing in state of the art, automated DNA based molecular analysis of a variety of chronic and infectious illnesses. MDL specializes and performs Polymerase Chain Reaction (PCR) with a larger menu of testing available in the field of infectious disease. Our main theme of research is in the field of Gynecology, Infectious Diseases, Infectious Arthritis, Tick-borne Diseases, Mycology, and Chronic Fatigue Syndrome (CFS).

Real-Time Reagents:

• Properly locate and identify stocks of primers and probes for each assay
• Properly locate and identify stocks of primers and probes for each assay
• Properly locate and identify plasmid stocks for each day
• Properly hydrate stock solutions of primer and probe for each assay
• Properly dilute positive standards from plasmid stock and aliquot for general laboratory use
• Run a test on each new batch of primer probe and standards compare to known standards to validate
• Keep all validation results cataloged for comparison to future
• Log all reagents made in appropriate places (rMDL inventory or log books)
• Log hydration numbers in the appropriate places

Bio-Plex Reagents:

• Properly locate reagents for the individual tests
• Using the appropriate buffers, dNTPs, primer mixes and taq to create the PCR mix for desired test
• Using the correct buffers, ASPE, dNTP, primer mixes and tsp to create the ASPE mix for the desired test
• Use stock primers to create primer mixes for the individual tests (PCR and ASPE)
• Use stock primers to create the Synthetic controls for individual tests
• Combine purchase bead for use in the clinical workflow
• Following procedure make any solutions needed to complete the assay procedures

Solutions:

• Following procedure locate components of solutions in the chemical room and create new mixtures (Digestive Additive, dNTPs, Pyro Washes, 5x loading Dye)
• After creating new reagents make sure they are tested and keep a log of when reagents are made tested and released to the clinical workflow.

Additional Responsibilities:

• Performs other similar or related duties as required or assigned.
• Assures activities are conducted in a safe manner and materials are handled according to safety guidelines.
• Assures customer privacy is maintained at all times.
• Participates as a team member and is accountable for own work responsibilities.
• Assures information is delivered in a manner that is supportive, timely and understandable.
• Initiative is demonstrated to proactively diagnose and resolve problems.
• Productive work habits are consistently displayed.
• Accuracy and quality are maintained for all activities.

Job Qualifications: General Knowledge, Skills, and Abilities (KSA’s) required

• 9 month Clinical Laboratory Technician training

Education and Experience:
Bachelor of Biology, Chemistry or related science field