Demo

Scientist II, Functional Genomics

Light Horse Therapeutics
San Diego, CA Full Time
POSTED ON 2/8/2025
AVAILABLE BEFORE 4/24/2025

Light Horse Therapeutics is a developer of first-in-class small molecule therapeutics based in San Diego, leading the way in precision genome editing applied to small molecule drug discovery and pioneering approaches to target the root causes of disabling and life-threatening diseases. Its advanced platform identifies cryptic chemically accessible functional domains within targets that play critical roles in disease biology. This high-throughput discovery platform systematically interrogates complex signaling pathways and uncovers unique sites of vulnerability in their native, cellular context to best ensure that discoveries in the lab translate into the clinic. Light Horse therapeutics was founded by leading academics Brian Liau, PhD (Harvard), Ben Cravatt, PhD (Scripps) and Nathanael Gray, PhD (Stanford) with financial backing from Versant Ventures and Mubadala Capital as well as three strategic investors : Bristol-Myers Squibb Co, AbbVie and Taiho Ventures.

Light Horse Therapeutics is seeking a talented, motivated, and creative Scientist II to join the Functional Genomics Group. The candidate will contribute to the design, execution, and analysis of CRISPR-based gene editing screens to study novel oncology targets. They will also perform mechanistic experiments to characterize these targets and the impact of various mutations on their function. The candidate will have the opportunity to employ multiple experimental techniques in cell biology to build an understanding of novel target biology in a dynamic start-up environment that employs cutting-edge tools in functional genomics and proteomic technologies.

Job Description :

  • Design and execute CRISPR-Cas9 mutagenesis screens using state-of-the-art technologies and multiple phenotypic readouts.
  • Design and execute mechanistic studies in disease-relevant preclinical models using both high-throughput and in-depth cell biology experiments.
  • Work closely with Computational Biology for the design and analysis of CRISPR screens and OMICs experiments
  • Keep abreast of relevant literature, and help strategize improvements to the gene editing platform and directions for target biology investigation
  • Position mostly requires benchwork responsibilities
  • Present results regularly at team meetings along with data interpretation and recommendations for next steps
  • Communicate science and strategy effectively in internal meetings and prepare written reports of data and literature analyses
  • Perform diligent and timely note-keeping

Required Qualifications :

  • PhD in biological science with postdoctoral and / or Biotech or Pharma research experience
  • Proficiency in mammalian cell line culture, especially large-scale projects
  • Experience with cell-based assays in high throughput format
  • Proficiency in standard molecular biology techniques, including DNA, RNA, and protein analysis, mammalian cell line transfection and lentiviral infection
  • Track record of being able to multitask while working independently in a lead capacity to create and drive projects
  • Excellent technical laboratory skills
  • Attention to details in documenting, executing, and analyzing experiments
  • Flexibility and eagerness to tackle new tasks and challenges, and meet aggressive timelines
  • Ability to work closely with team members and across disciplines
  • Preferred Qualifications

  • Experience with CRISPR-Cas9 mutagenesis screens
  • Experience with lentivirus production and infection and selection
  • Experience constructing next generation sequencing libraries and analysis / interpretation of genomics data
  • Experience with parallelized high throughput assay development for cell growth and gene / protein expression
  • Experience in tools assessing the transcriptional and epigenetic regulation of genes is a plus
  • Experience with flow cytometry and / or protein / protein interactions is an advantage
  • We offer a competitive total compensation package, as well as working in a supportive team environment.

    The anticipated salary range for candidates who will work in San Diego, CA is $116,000 to $160,000 . The final salary offered to a successful candidate will be dependent on several factors that may include but are not limited to the type and length of experience within the job, type and length of experience within the industry, education, etc.

    Salary : $116,000 - $160,000

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