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Genotyping Research Associate II

Ohalo
Mills, NC Full Time
POSTED ON 4/23/2025
AVAILABLE BEFORE 5/22/2025
Position: Research Associate II - Genotyping
Location: Mills River, NC
Type: Full time
Opportunity: Ohalo Genetics is seeking a motivated Genotyping Research Associate II to join our high-throughput genotyping team.
This position will focus on executing established genotyping workflows, including using Next Generation Sequencing (NGS) technologies and molecular biology techniques.
The successful candidate will have a Bachelor's degree in a related field with 3 years experience or a Master's degree with more than 1 year relevant experience.
This is a dynamic, hands-on role suited for individuals with a strong background in PCR, Illumina sequencing, and molecular biology workflows.
Working closely with other research associates and scientists, the successful candidate will play a pivotal role in screening large sets of plant tissue samples and will contribute to the successful execution of our high throughput genotyping pipeline essential to Ohalo Genetics' production objectives.
Responsibilities:
  • Perform DNA extraction, PCR, and sequencing library preparation.
  • Work with other scientists to develop and test new, customized genotyping workflows, including marker-based and whole-genome genotyping approaches.
  • Communicate genotyping timelines, updates, and results with related teams.
  • Carefully execute biotechnology/molecular biology protocols.
  • Perform analytical assays for QC or research purposes.
  • Maintain detailed lab records in an electronic notebook and ensure accurate data entry.
  • Monitor equipment and minimize downtime in the genotyping pipeline.
  • Supervise and support junior RAs in the team as needed.
Candidate Profile:
  • Bachelor's or Master's degree in Biology, Plant Genetics, Molecular Biology, Biochemistry, or a similar field.
  • 3 years in a relevant role (or 1 with a Master's).
  • Strong skills in molecular biology techniques, including DNA and RNA extraction, PCR, qPCR, or ddPCR.
  • Proficiency in Illumina sequencing, library preparation, and QC.
  • Detail-oriented with strong organizational and communication skills.
  • Familiarity with plant breeding, genetic engineering, and plant tissue culture/regeneration.
  • Ability to work independently and collaboratively in a fast-paced environment.
  • Proficiency in MS Word and Excel.
  • A growth mindset with a commitment to continuous improvement.
Desired Skills/Experience:
  • Experience designing and optimizing complex PCR workflows.
  • Advanced Illumina library preparation and sequencing knowledge.
  • Experience with automated liquid handling equipment.
  • Familiarity with delicate analytical instruments, such as Flow Cytometer, Nanodrop, Bioanalyzer, or Microplate Reader.
  • Demonstrated process optimization and troubleshooting abilities.
  • Basic supervisory experience or proven leadership skills.
  • Advanced data analysis skills using R or similar tools.
Demands:
  • On-site (one location) Monday through Friday
  • Standing 40-50% of the day
  • Intensive pipetting requiring dexterity and vision

If you are passionate about molecular biology, plant genetics, and advancing genotyping technologies in a collaborative, startup environment, we encourage you to apply. We seek to transform breeding and production systems with a goal of helping to sustainably support the world's growing population.

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