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RCI-ABBV-28513 Associate Scientist (Immunofluorescence Staining/Mammalian Cell Culture/RNA Extractio

Rangam
North Chicago, IL Full Time
POSTED ON 1/23/2025
AVAILABLE BEFORE 4/21/2025

100% on site

Position Title : Research Technician in Cell and Molecular Biology

Can be level II or III depending on experience

Length of Contract : 6 months

  • A Research Technician position in Cell and Molecular Biology is available at the Genomic Research Center (GRC) at client.
  • We are seeking a highly motivated research technician to work independently and collaboratively with other scientists on projects involving human-induced pluripotent stem cell (hiPSC) reprogramming, cell differentiation, CRISPR editing, high-throughput functional screening, and a variety of molecular biology experiments in the wet lab.
  • The successful candidate will be responsible for performing time-sensitive experiments and supporting extended hiPSC differentiation protocols, both of which require daily attention to detail.

The applicant is expected to :

  • Conduct routine experimental protocols and procedures in the wet lab.
  • Maintain tissue culture, including stem cells and stem cell-derived cells.
  • Accurately record experimental setups, SOP protocols, and data quality control.
  • Maintain efficient and continuous communication with the manager and team members.
  • Support team members with routine cell culture procedures, including media preparation and reagent aliquoting.
  • Conduct molecular and cell biology experiments such as qRT-PCR, immunofluorescence, and Western blot analyses.
  • Qualifications

  • A bachelor’s or master’s degree in biochemistry or molecular cell biology, with at least three years of hands-on laboratory experience.
  • Trained and experienced in using aseptic techniques in a laminar flow biosafety cabinet.
  • Significant cell culture expertise, including maintenance, freezing, thawing, and passaging.
  • Proficiency in biochemical assays such as Western blot, immunofluorescence, and flow cytometry.
  • Skilled in molecular biology techniques such as RNA extraction, cDNA synthesis, and RT-qPCR.
  • Knowledge of serial dilution and calculation of solution concentration.
  • Excellent written and oral communication skills.
  • Organized in planning long experimental protocols that may sometimes require working on weekends.
  • Nice to have :

  • Experience with culturing human iPSC and differentiating iPSC into brain cell types.
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