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Laboratory Research Technician - Moatti lab

University of Pittsburgh
Pittsburgh, PA Full Time
POSTED ON 2/1/2025
AVAILABLE BEFORE 3/2/2025
Performs a variety of independent laboratory work and experiments such as determining experimental protocols and preparation of specimens and cultures. Prepares reports of findings subsequent to compilation and analyzation. Oversees specimen inventory. Develops documentation for publication; crafts manuscripts and grant proposals.

Med-Otolaryngology

Required Education And Experience

Appropriate BSC in Cell Biology, Molecular Biology, Biomedical Engineering or a related field.

Required Knowledge, Skills, And Abilities

Cell biology skills:

  • Cell culture 2D & 3D

Animal Skills

  • Comfortable with Handling animals
  • Comfortable with IP/IV injection
  • Comfortable with Survival surgery

Excellent written and oral communication skills

Ability to work independently and collaboratively

Preferred Skills

Molecular biology skills:

  • RNA extraction
  • PCR
  • RNA sequencing
  • Extracellular vesicles
  • cAMP assay
  • IP3 assay
  • Mass spectrometry
  • Plasmid preparation
  • Stem Cells
  • Metabolomics
  • Data analysis
  • Organ of Corti dissection
  • Perilymph sampling
  • Image processing
  • Leadership and project management skills

Essential Functions

  • Cell culture
  • liposome modifications
  • extracellular vesicles isolation and loading
  • Histology and tissue processing including fixation (perfusion and immersion)
  • inner ear gross and fine dissection
  • tissue clearring
  • sectioning
  • immunohistochemistry and fluorescent imaging
  • Accurate and detailed record keeping in electronic lab notebooks.
  • In vivo delivery to the inner ear of pigs which might involve using adeno-associated viral vectors and nonviral vectors
  • Assays of physiological (auditory brainstem response) and behavioral thresholds for hearing function before and after herapy in animal models.
  • RNA Sequencing
  • Mass spectrometry
  • protein purification
  • HPLC
  • Handling human and animal samples

Physical Effort

No heavy physical work. Must be able to sit for extended periods of time and to stand and move about as necessary. Excellent vision and manual dexterity essential for performing job tasks.

The University of Pittsburgh is committed to championing all aspects of diversity, equity, inclusion, and accessibility within our community. This commitment is a fundamental value of the University and is crucial in helping us advance our mission, which includes attracting and retaining diverse workforces. We will continue to create and maintain an environment that allows individuals to discover, belong, contribute, and grow, while honoring the experiences, perspectives, and unique identities of all.

The University of Pittsburgh is an Affirmative Action/Equal Opportunity Employer and values equality of opportunity, human dignity and diversity. EOE, including disability/vets.

Assignment Category

Full-time regular

Job Classification

Staff.Laboratory Research Technician III

Job Family

Research

Job Sub-Family

Laboratory

Campus

Pittsburgh

Minimum Education Level Required

Bachelor's Degree

Minimum Years Of Experience Required

2

Will this position accept substitution in lieu of education or experience?

Combination of education and relevant experience will be considered in lieu of education and/ or experience requirement.

Work Schedule

Monday - Friday, 8:30 a.m. - 5:00 p.m.

Work Arrangement

On-Campus: Teams that work on campus, in an office, or in a lab.

Hiring Range

TBD Based Upon Qualifications

Relocation Offered

No

Visa Sponsorship Provided

No

Background Check

For position finalists, employment with the University will require successful completion of a background check

Child Protection Clearances

Not Applicable

Required Documents

Resume

Optional Documents

Cover Letter, Writing Sample

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