What are the responsibilities and job description for the DNA/RNA Extraction and qPCR Preparation Internship position at Zintellect?
The Air Force Research Laboratory 711 Human Performance Wing (HPW) is offering an internship at Wright Patterson Air Force Base.
What will I be doing?
As an Oak Ridge Institute for Science and Education (ORISE) participant, you will join the genomics core team of scientists and researchers in an effort to impact scientific research by enabling a more comprehensive understanding of an individual's genetic information, leading to new discoveries and advancements in the field of human performance. This team specializes in the field of Genomics, Transcriptomics and Epigenetics. This combination of ‘omics provides a more complete understanding of an individual's genetic makeup and regulation, whereas a targeted approach can be taken to focus on one of these areas.
- Enhance skills in DNA and RNA extraction using a variety of cell samples and whole blood samples
- Optimize extraction protocols, maintaining accurate records and monitoring quality standards
- Gain experience in quantification and quality control using use spectrophotometers, fluorometers, and electrophoresis (e.g., Agilent TapeStation, Agilent Bioanalyzer, Qubit) to assess the quantity, purity, and integrity of nucleic acids.
- Participate in nucleic acid preparation for qPCR assays, troubleshooting protocols for cDNA conversion and qPCR preparation and minimizing cross-contamination
- Collaborate and communicate with research teams and project leaders to meet project-specific needs and adapt protocols based on project goals.
- Participate in data management and documentation, maintaining detailed and organized records of all procedures, experiments, and sample tracking, ensuring traceability from sample extraction to qPCR data analysis.
- Support generation of reports summarizing DNA/RNA extraction quality, qPCR preparation success, and sequencing readiness.
Where will I be located?
Dayton, Ohio
What is the anticipated start date?
Exact start dates will be determined at the time of selection and in coordination with the selected candidate. Applications are reviewed on an ongoing basis and internships will be filled as qualified candidates are identified.
What is the appointment length?
This appointment is a twelve-month research appointment, with the possibility to be renewed for additional research periods. Appointments may be extended depending on funding availability, project assignment, program rules, and availability of the participant.
What are the appointment provisions?
You will receive a stipend to be determined by AFRL. Stipends are typically based on a participant’s academic standing, discipline, experience, and research facility location. Other provisions may include the following:
- Health Insurance Supplement (Participants are eligible to purchase health insurance through ORISE)
- Relocation Allowance
- Training and Travel Allowance
About AFRL
The 711th Human Performance Wing (711 HPW), headquartered at Wright-Patterson Air Force Base in Ohio, is the first human-centric warfare wing to consolidate human performance research, education, and consultation under a single organization. Established under the Air Force Research Laboratory (AFRL), the 711 HPW is comprised of the Airman Systems Directorate (RH) and the United States Air Force School of Aerospace Medicine (USAFSAM). For more information about the Air Force Research Laboratory, 711 Human Performance Wing, Airman Systems Directorate, Airman Biosciences Division, please visit https://afresearchlab.com/.
About ORISE
This program, administered by Oak Ridge Associated Universities (ORAU) through its contract with the U.S. Department of Energy (DOE) to manage the Oak Ridge Institute for Science and Education (ORISE), was established through an interagency agreement between DOE and DoD. Participants do not enter into an employee/employer relationship with ORISE, ORAU, DoD or any other office or agency. Instead, you will be affiliated with ORISE for the administration of the appointment through the ORISE appointment letter and Terms of Appointment. Proof of health insurance is required for participation in this program. Health insurance can be obtained through ORISE. For more information, visit the ORISE Research Participation Program at the U.S. Department of Defense.
