What are the responsibilities and job description for the AAV PRODUCTION SENIOR RESEARCH ASSOCIATE / ASSOCIATE SCIENTIST position at Amber Bio?
Amber Bio is a biotechnology company pioneering new gene editing modalities using multi-kilobase edits to reach previously undruggable patient populations. Founded by pioneers in the CRISPR field from leading institutions for gene editing research, the company is developing a first-of-its-kind RNA writing platform that can correct thousands of bases at once, thereby correcting genetic mutations safely and reversibly. Multiple openings are available on a rapidly growing team. As an early team member, you will have the opportunity to contribute to strategic planning and future hiring. If you are interested in building a new frontier in genetic medicine, please apply via LinkedIn or through our website ( www.amber.bio ). We are seeking a skilled AAV Production Senior Research Associate / Associate Scientist to join our growing team. This role involves leading and executing all aspects of AAV production, with a focus on downstream optimization to enhance viral yield, quality, and consistency. The ideal candidate will bring extensive expertise in AAV production across adherent and suspension cell platforms and demonstrate a proven track record of optimizing protocols for scale-up, purification, and workflow efficiency. This role requires a collaborative spirit and the ability to work across teams to support AAV production and characterization. Key Responsibilities AAV Production and Process Development Develop and scale AAV production processes, including IOX gradient purification and affinity column purification, for increased yield and optimized turnaround. Perform cell culture for AAV production (adherent and suspension). Operate bioreactors, AKTA systems, pumps, mixers, and automated buffer exchange systems to streamline and automate purification workflows. Continuously improve purification methods (e.g., AKTA tandem purification) to automate and streamline multi-sample processes. AAV Characterization and Assay Development Run AAV potency, biodistribution, transduction efficiency, and immunofluorescence assays to evaluate AAV performance in mammalian cell lines. Perform ddPCR, sequencing, and ELISA-based assays for quantitative and qualitative characterization of AAV vectors, including titer, purity, and genome integrity assessments. Conduct in-depth analysis on AAV stability, aggregation, and particle-to-infectivity ratios to refine vector quality and consistency Collaborate on optimizing purification and buffer exchange protocols to enhance yield, purity, and formulation stability across multiple AAV serotypes. Support upstream cell culture scale-up with a focus on consistent AAV quality and reproducibility for downstream characterization assays. Inventory, Documentation, and Workflow Management Manage lab inventory, ensuring timely ordering of supplies and efficient use of resources. Maintain accurate records and data using platforms such as Benchling Workflow; author SOPs, testing summaries, and production batch records. Produce AAV lots to support research and preclinical studies, ensuring all production steps meet quality and consistency standards. Collaboration and Training Train and guide team members in AAV production techniques. Support viral transduction for in vitro and in vivo applications. Collaborate with cross-functional teams to meet project goals, and advance novel vectors into pipeline. Lead troubleshooting efforts for production challenges and ensure compliance with established protocols. Qualifications Education : Bachelors or Masters in Molecular Biology, Biochemistry, Virology, or a related field, or equivalent experience. Experience : 4 years of hands-on industry experience in AAV production, preferably with both adherent and suspension HEK293 cells. Proven track record in scale-up production and purification processes. Technical Skills : Proficiency with AKTA, bioreactors, molecular biology techniques (DNA / RNA / protein isolation, cDNA synthesis, RT-PCR, qPCR, digital PCR, western blot, and immunofluorescence). Leadership : Demonstrated ability to manage and mentor team members in a production environment. Analytical Skills : Strong troubleshooting and optimization skills for viral vector production. Preference will be given to those who display : High motivation, with a strong work ethic and dedication to generating impact Attention to detail, with the ability to extract deep insights from data Ability to go from ideation to data in an independent fashion Long-term personal vision with defined career goals High EQ with team-oriented thinking Demonstrated excellence in small team environments, including a no task is too small attitude If you have a passion for advancing gene editing technologies and desire to be part of a pioneering biotech company, we encourage you to apply and join our ambitious team. Please apply directly through LinkedIn or through our website ( www.amber.bio ). Amber Bio is an equal-opportunity employer and encourages applications from candidates of diverse backgrounds. We value diversity and are committed to creating an inclusive and supportive work environment for all employees.
