The Perfect Addition to Our Team

You are a versatile, self-motivated, and eager Research Associate I who is looking forward to rolling up your sleeves and contributing to all aspects of the CMC upstream team. You thrive in a fast-paced environment and able to juggle competing priorities. You work successfully in a team environment with strong interpersonal skills to effectively build relationships with cross functional groups. 

The Opportunity

The Research Associate I will support high-throughput screening, microbial/yeast fermentation, and primary recovery. They will be responsible for the high-throughput screens to support construct screening and fermentation/cell culture, as well as purification to generate materials for in vitro and in vivo studies. The ideal candidate will work side-by-side with biologics discovery, downstream, analytical, and pharmacology team members to identify the lead candidate and nominate candidate selections.

Responsibilities

• Set-up, inoculate, and operate meticulously shake flasks, small- and medium-scale mammalian cell culture, yeast and bacterial fermentations.
• Run deep well plates, shake flask for construct screening and process optimization.
• Perform primary recovery operations such as homogenization, centrifugation, inclusion body wash, solubilization, depth filtration, and refolding.
• Run SDS-PAGE gels, western blots, and protein concentration assay.
• Operate AKTA Avant, Bio-Rad NGC FPLC systems for protein purification (as needed)
• Maintain upstream lab equipment, lab space, inventory of upstream raw materials and consumables.
• Record experimental details and observations in the laboratory notebooks in a timely manner.
• Present experimental design, data, and research findings in internal team meetings.

The Necessities

At Entrada, our passion for science, our devotion to patients and our values drives our behavior:

• Humanity - We genuinely care about patients and about one another.
• Tenacity - We are relentless and persistent in the pursuit of developing therapies for patients.
• Creativity - We are creative problem solvers.
• Collaboration - We are more than the sum of our parts.
• Curiosity - We have a growth mindset and push conventional thought and theory.

To thrive on our team, you will need to come with:

• BS or MS degree in biochemical engineering, biotechnology, biochemistry, or a related field with at least 2 years of wet lab experience in an academic or industry environment.
• Experience working with E. coli or/and Pichia pastoris.
• Experience running SDS-PAGE and western blots for monitoring protein production.
• Experience with primary recovery operations such as centrifugation, depth filtration, homogenization, solubilization, refolding.
• Experience with TFF operation to concentrate and diafilter recombinant proteins (a plus).
• Strong theoretical understanding of the principles of fermentation/cell culture process.
• Strong communication skill (oral and written) and ability to work effectively across functions.
• Ability to proactively prioritize and help drive experimental designs forward.
• Growth mindset with ability to adapt to changing priorities.
• Able to work during outside of regular business hours and weekends.