Job Title: Biochemist/Biophysicist

Location: Louisville, CO, Onsite

Duration: 12 Months Contract (Possible conversion)

Schedule: Business shift and hours

Job Description:

This position is an opportunity to learn and grow within a dynamic chemical biology team that supports early molecule discovery. Our multidisciplinary team combines medicinal chemistry, biophysics, structural biology, and enzymology to identify molecular leads that result in targeted oncology therapeutics.

We are seeking a Biochemist/Biophysicist who will contribute to the early molecule discovery team, in both bioconjugation as well as biophysical characterization.

Responsibilities of this position include producing a variety of customized antibody-drug conjugates, optimizing the conjugation and purification procedures for a variety of biologics and linkers, performing bioanalytical analyses on the resulting conjugates, and biophysically characterizing protein-protein and protein-ligand interactions.

Responsibilities:

• Work closely with senior scientists to accomplish team objectives and research milestones.

• Contribute to the development and optimization of conjugation schemes to achieve the required product specifications.

• Commit to producing and maintaining the highest standard of quality in antibody-conjugate productions.

• Perform the following bioconjugation procedures

1) Antibody and recombinant protein isolation and purification,

2) Purification of antibodies and conjugates using various liquid chromatography methods (IEX, SEC, HIC).

3) Antibody and conjugate validation via various functional tests,

4) Biophysical characterization of proteins and conjugates (e.g., UV/VIS spectrophotometry, DLS, SEC-MALS, native mass-spectrometry Tm, or Tagg determination).

• Characterize protein-ligand and protein-protein interactions by a variety of methods including but not limited to; Intact protein and peptide-based mass spectrometry, native mass spectrometry, fluorescence spectroscopy, SPR, BLI, and DSF.

• Biophysically characterize protein samples destined for multiple workflows.

• Learn and develop technical expertise in protein biochemical and biophysical characterization across multiple platforms which may Client outside their current skill set.

• Develop and continually advance one’s knowledge base on genetically targeted drugs and structure-based drug design.

• Adapt to highly dynamic project priorities, with diligent and rapid communication of scientific results and insights to the team, including maintaining an ELN and uploading data to the database.

• Perform work in compliance with the Client’s Research Policies.

The physical demands of this job are consistent with lab duties (e.g. the ability to move solvent and waste bottles, stand in a chemical hood for extended periods, gather packages received, etc.). This position’s work environment is based primarily in a wet lab, with some data analysis and documentation in an office environment.

Qualification:

• BS, MS, or PhD in Biochemistry, Chemical Biology, or related field.

• 2 years of hands-on experience in biophysics, bioconjugation, protein labeling, or a combination thereof.

• Direct knowledge in assay development and utilizing SPR/BLI (e.g. Biacore or Octet) or LCMS technologies (e.g. QTOF or Orbitrap)

• Highly organized with excellent analytical, documentation, and multi-tasking skills.

• Proven ability to work in a flexible, team-oriented environment.

• Working knowledge of chemical labeling methods and technologies.

• Experience in method or assay development utilizing a variety of LCMS technology (e.g. QTOF or Orbitrap).

• Working knowledge of fluorescent-based method or assay development (e.g. FP, FRET, etc).

• Working knowledge in assay development and utilizing SPR/BLI (e.g. Biacore or Octet).

• Excellent interpersonal skills with the ability to communicate and interact well with colleagues at all levels.

• Committed to staying current with the latest scientific breakthroughs in structure-based drug design and biophysical analysis.