Job Title: Sr. Associate Scientist or Scientist, RNA Process Development

Location: Seattle, WA * 100% Onsite

Duration: 12 Months

Pay range: $60-$68/hr on W2

Top Skills:

• Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
• Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
• Experience working with, storing, and analyzing purified nucleic acids and proteins
• Experience with optimizing molecular cloning reactions
• Experience with gene editing primary human cells or immortalized cell-lines

Description

• Seeking a highly motivated Sr. Associate Scientist, or Scientist, to work in a fast-paced PD environment applying rigorous biochemical principles towards developing and characterizing ligated oligonucleotides for gene editing applications. As a part of the Gene Delivery and Editing Process Development (GDEPD) organization, the ideal candidate will possess sufficient experience with handling, manipulating, and characterizing in vitro produced RNA oligomers (50 – 200 nt).
• A successful scientist will directly employ molecular cloning, biophysical and analytical tools to develop our next-generation gene edited therapies through the development of RNA oligomers. An outstanding candidate will be driven by a desire to create, optimize, and define processes with speed in a collaborative environment.

Key Responsibilities:

• Design and execute nucleic acid ligation reactions using “splint ligation” methodology
• Design and execute ion-pairing (IP) reversed-phase (RP) chromatography methods
• Perform purification of oligonucleotides using chromatography and/or preparative PAGE
• Design and evaluate the performance of new biophysical assays to identify and assess CQA’s
• Manage samples and data related to gene editing platform process development
• Provide experimental design and executional support to team members in adjacent and related functions (e.g. clinical development programs, high throughput process development, etc.)
• Actively participate and support lab maintenance and continuous improvement initiatives
• Maintain accurate and detailed laboratory notebook and documentation
• Summarize and present data in group meetings and contribute to group discussions
• Author technical reports, study memos, and protocols

Basic Qualifications:

• STEM degree with 2 years of relevant industry experience; title/compensation will be commensurate with education, experience, and skillset
• Proficiency with biochemical techniques to analyze oligonucleotides (PAGE and CE)
• Proficiency with analytical IP-RP chromatography for RNA (including mRNA) purity
• Experience working with, storing, and analyzing purified nucleic acids and proteins
• Independently motivated with the ability to work in teams
• Ability to communicate clearly and concisely through oral presentation and technical writing
• Ability to leverage literature, internal and external resources to solve problems and develop new technologies
• Possess a growth mindset and the ability to thrive in a fast-paced, highly collaborative environment

Preferred Qualifications:

• Degree in biological or chemical engineering, biochemistry, or bio-related field
• Experience with optimizing molecular cloning reactions
• Experience with gene editing primary human cells or immortalized cell-lines
• Experience with solid-phase oligonucleotide synthesis
• Experience with bioinformatic tools used to predict RNA secondary structure
• Experience with JMP for DoE design and analysis