The Division of Hematology Oncology is seeking a Staff ResearchAssociate 1 with experience in a BSL2 laboratory. In this role, you willperform tissue culture work; maintain different cell lines and human cells;perform large-scale selection of different populations of cells; grow andpurify viral vectors (transfection, purification, titration) and live virus;maintain log of vectors, virus, and cells. Process blood and tissue samples(mouse, human, primate) and sort for selected populations of cells. HIVinfection of cultured cells and human cells. Perform molecular biology: DNA andRNA extraction, molecular cloning, PCR and RT-PCR.

You will be expected to maintain a laboratory notebook in enoughdetail that results can be reproduced. Plan experiments in conjunction withsupervisor; but, also must be able to analyze and interpret data and planexperiments independently. Additionally, you will work closely with other techniciansin conducting large-scale experiments; assist in training of undergraduates;order, stock, and maintain inventory on laboratory supplies; and assist withhandling of mice. You will perform animal handling and manipulations - varioustypes of injections, retro orbital bleeds, biopsies, euthanizations, and sampleharvesting (blood, various tissues). Assist with humanized mouse surgeries andmonitor mouse health and cell engraftment for downstream applications.

Hourly Salary Range: $25.75 - $30.71

Required:

• Bachelor's degree or higher in biology or related field. Workingknowledge and experience with basic biology.
• Skill in using BSL2 laboratory practices and sterile techniques.
• Skill in preparing media and reagents used in tissue culture work.
• Skill in handling human cells and cell lines (adherent andsuspension). Skill in counting cells, freezing/thawing cells, storing cells,passaging cell lines, maintaining primary cells.
• Skill in performing vector transfection, transduction, andtitration.
• Skill in flow cytometry staining of samples (cells, blood,tissue).
• Skill in performing DNA/RNA extractions, viral RNA, PCR, RT-PCR.
• Willing to work long hours (planned or unplanned), overtime, oralternate hours (such as weekends, offset hours and late at night) asrequested.
• Skill in performing a given protocol in a reproducible way. Skillin reading laboratory protocols and other scientific documents. Skill introubleshooting and optimizing protocols as necessary, documenting any protocoldeviations with thorough explanation of why deviation occurred.
• Ability to work independently to finish tasks in a timely manner.Ability to set priorities and manage time effectively despite unanticipated frequentinterruptions/distractions. Ability to adjust to changes in the workload andswitch focus as needed. Ability to make sound judgments and decisions. Veryorganized with meticulous attention to detail. Skill in detailed documentationof activities, tasks, progress, observations, etc.