Demo

Bench Scientist

Synectics
Media, PA Full Time
POSTED ON 1/29/2025
AVAILABLE BEFORE 4/28/2025

Description

  • Will be part of a team to differentiate and characterize cellular models derived from human induced pluripotent stem cells (iPSCs), and to perform genetic perturbations (KD, KO, KI, overexpression) in iPSC-derived cells for drug discovery applications :
  • Maintain iPSC cell culture, perform differentiate and QC characterization in supporting assay development and translational studies.
  • Perform detection of biomarker changes using various assay methods, such as ELISA, Western, imaging, RT-PCR.
  • Utilize various transfection / transduction methods to knockdown or overexpress target genes in differentiated cells.
  • Analyze and summarize experimental data using a variety of laboratory instrumentation and computer software's.
  • Maintain accurate laboratory notebooks according to company guidelines, and present data to colleagues or in team meetings.
  • Primary tasks will include large scale DNA purification and QC, expression vector preparation for large scale transient transfections, confirmatory DNA sequence analysis, and plasmid design and construction.
  • Expected to maintain accurate and organized records and to prepare archiving documents.

Qualifications :

  • BS or MS in Biological Sciences required with laboratory experience in molecular biology and cell biology.
  • Has theoretical and practical scientific knowledge in biological sciences equivalent to a BS degree with hands on laboratory experience.
  • Experience in sterile cell culture and gene? Knockdown and overexpression) techniques.
  • Experience in cell-based assays (e.g. immune-sandwich, reporter gene etc.) and / or biochemical assays.
  • Ability to execute experiment independently and work collaboratively with colleagues.
  • Able to do independent literature research, analyze, summarize and interpret experimental data and maintain records according to company policy.
  • Ability to cooperate with others both to accomplish scientific goals and to perform general maintenance duties.
  • Previous working experience on iPSC differentiation, viral delivery vectors, and / or FACS cell analysis is preferred.
  • Preferred knowledge of molecular biology techniques including microbial cell culture, plasmid DNA purification, DNA subcloning, restriction enzyme analysis, DNA sequencing, expression plasmid construction and experience in the use of molecular biology design software (Lasergene, Clonemanager, and Vector NTI).
  • Benefits :

    Healthcare Insurance : Synectics offers eligible employees and their dependents healthcare coverage through BlueCross BlueShield of Illinois. Eligibility begins on the 1st day of the calendar month following 60 days of continuous full time employment with Synectics. Premiums are subsidized by Synectics.

    Dental Insurance : Synectics offers eligible employees and their dependents a dental plan through MetLife. Eligibility begins on the 1st day of the calendar month following 60 days of continuous full time employment with Synectics.

    Vision Insurance : Synectics offers eligible employees vision insurance through VSP. Eligibility begins on the 1st day of the calendar month following 60 days of continuous full time employment with Synectics.401(k) Plan : The Synectics Inc. Investment Savings Retirement Plan. Synectics offers all employees who are 21 years of age or older the opportunity to invest in the 401(k) Plan on the first enrollment date that is at least 30 days after employment begins. Enrollment dates are each January 1st, April 1st, July 1st, and October 1st.

    Technical Certification Bonus : Synectics is pleased to award its employees a bonus of up to $500 for an approved professional certification. In determining the bonus amount, Synectics will consider the cost of the test(s) for any certification relating to your current position, achieved during your employment with us. Only one Certification Bonus per calendar year may be awarded per employee. Only current, active employees will be eligible to receive this bonus. It will be awarded 90 days after the Synectics office has received documentation confirming the successful completion of the certification.

    Synectics is an equal opportunity employer.

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